Cholesterol metabolism: use of D2O for determination of synthesis rate in cell culture.
نویسندگان
چکیده
Cholesterol synthesis in cell culture in the presence of D2O yields a spectrum of enriched molecules having a relative abundance that indicates random substitution of deuterium for hydrogen. Quantitation of the absolute rate of cholesterol synthesis is obtained by isotope ratio mass spectrometry. Mevinolin and 26-hydroxycholesterol both decrease cholesterol synthesis rate but have a discordant effect on HMG-CoA reductase activity.
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عنوان ژورنال:
- Journal of lipid research
دوره 26 8 شماره
صفحات -
تاریخ انتشار 1985